Full Project Title: Conservation of insectivorous microbats; new approaches to mapping genetics & infectious disease threats in Aust global biodiversity hotspot
Aim: Determine the genetic structure of microbat populations.
- Map viral pathogens which may pose a threat to bat populations.
- Identify potential disease risks for humans in areas where interactions with bats may occur.
- Blood and swab samples screened for infectious diseases.
Information will be used to inform species management and public health risks for organisations working in the region.
In June 2017 we co-organised a bat night together with Murdoch University outreach team and Kanyana Wildlife Rehabilitation Centre. The focus was on the remarkable flight adaptations of bats and introduced the community to microbats species present in Mt Gibson Sanctuary and Charles Darwin Reserve.
First fieldwork season is completed. This included trapping in areas managed by Bush Heritage Australia, the Australian Wildlife Conservancy and crown land. Over 300 samples were taken across 11 species at 6 sites, additional trapping sites were identified in preparation for the second fieldwork season in 2018.
All samples were screened for Australian bat lyssavirus (ABLV). We have also implemented a pan-lyssavirus assay designed to detect the full range of lyssaviruses found globally rather than just focussing on the insectivorous-associated ABLV. This limited sample set has been found to be negative for lyssavirus. We have implemented targeted tests for the detection of viral groups of interest including coronaviruses, paramyxoviruses, adenoviruses and astroviruses.
We have employed next generation sequencing for pathogen discovery (a non-targeted approach) on faecal samples from Gould’s wattled bats captured at Mt Gibson Sanctuary and Charles Darwin Reserve. While only a small amount of this data has been analysed, amongst some of the interesting results is the huge amount of insect and plant viruses in the bat faeces, a by-product of their diet.
During October 2017 the Australian Animal Heatlh Laboratory at CSIRO will be screening all collected blood samples for antibodies to viral families of significance. This type of testing complements the targeted and non-targeted approaches, as we can determine whether an animal has been exposed to the virus in the past but not currently carrying it.
The project has recruited a 4th year veterinary student to study the prevalence of the blood parasite Trypanosoma ssp in blood samples (by PCR detection) and blood smears (for morphologic identification of the parasite itself). We have screen 54 bats by PCR which have a trypanosome prevalence of 46% and we will be screening the additional samples during October 2017.
- OrganisationMurdoch University